ITC
Drug Discovery & Development
Assessing Biological Activity During Protein Engineering
Antibodies, antibody-like proteins, and other biotherapeutics represent a large and growing number of molecular entities entering human clinical trials for virtually all disease indications. Different analytical methods are employed to monitor the biological activity of engineered therapeutics to confirm that proteins remain functionally active going into development.
Isothermal Titration Calorimetry (ITC) provides a quick and easy way to monitor the biological activity of engineered proteins. Activity is assayed by monitoring both the binding of engineered proteins to their therapeutic targets and the stoichiometry of binding to ensure that a biotherapeutic retains functional activity. Using ITC, biological activity is monitored in solution, label-free, requiring no immobilization and minimal assay development, thus providing unique benefits over traditional methods.
Why use ITC to monitor biological activity?
- All measures are done in solution, label-free, requiring no immobilization and minimal assay development.
- Monitor both binding affinity and stoichiometry in a single experiment
- Quickly and easily assess whether engineered protein is active.
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