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iTC₂₀₀ Isothermal Titration Calorimetry

The iTC₂₀₀ system is the most sensitive isothermal titration calorimeter available.  It is designed to address the needs of today’s life science researchers – particularly those engaged in drug discovery and development.  With its reduced size and associated sample quantity requirements, the iTC₂₀₀ is significantly faster than previous models.  Designed for ease-of-use, all functions of the iTC₂₀₀ are operated through software to facilitate fast and accurate analyses.

Applications include:

• Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
• Lead optimization.
• Enzyme kinetics.
• Assessment of the effect of molecular structure changes on binding mechanisms.
• Assessment of biological activitiy.

Why iTC₂₀₀?

• More than just affinities:  Simultaneous determination of all binding parameters in a single experiment - information unobtainable from more limited binding assays.
• Directly measure sub-millimolar to nanomolar binding constants (10² to 10⁹ M^-1).  Measure nanomolar to picomolar binding constants (10⁹ to 10¹² M^-1) using the competitive binding technique.
• Application versatility:  Investigate any biomolecular interaction with high sensitivity.  The sample cell is 200 µL. As little as 10 µg of protein can be used.
• True in-solution technique: No labeling or immobilization required. No buffer restrictions. Easily handles turbid solutions.
• Easy to use:  Includes user friendly experimental design wizards and easy filling and cleaning procedures.
• Fast time to first result:  With fast equilibration times, up to two runs per hour can be easily accomplished.  Requires no assay development or dedicated user to achieve high quality results.
• Automatable:  iTC₂₀₀ can be upgraded to a fully automated version with a sample throughput of at least 75 samples per day with a capacity to process as many as 384 samples unattended.

iTC₂₀₀ features:

• Active cell volume ~200 µL.
• Non-reactive Hastelloy® cells for excellent chemical resistance.
• Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance.
• Precision liquid delivery system for accurate and reproducible injections
• User-selectable mixing speeds to match sample conditions.
• Three user selectable response times (U.S. Patent number 5,967,659) for application versatility.
• Operating temperature range of 2ºC to 80ºC.
• Peltier controlled for rapid temperature equilibration.
• Includes the iTC₂₀₀ Washing Module for easy cell and syringe filling and  cleaning.

Need more information?  Contact us.

Journal Citations for iTC₂₀₀ and Auto-iTC₂₀₀